Biphasic Oxygen Kinetics of Cellular Respiration and Linear Oxygen Dependence of Antimycin A Inhibited Oxygen Consumption

Authors: Hütter E.¹; Renner K.²; Jansen-Dürr P.¹; Gnaiger E.³

Source: Molecular Biology Reports, Volume 29, Numbers 1-2, 2002 , pp. 83-87(5)

Publisher: Springer

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Abstract:

Oxygen kinetics in fibroblasts was biphasic. This was quantitatively explained by a major mitochondrial hyperbolic component in the low-oxygen range and a linear increase of rotenone-and antimycin A-inhibited oxygen consumption in the high-oxygen range. This suggests an increased production of reactive oxygen species and oxidative stress at elevated, air-level oxygen concentrations. The high oxygen affinity of mitochondrial respiration provides the basis for the maintenance of a high aerobic scope at physiological low-oxygen levels, whereas further pronounced depression of oxygen pressure induces energetic stress under hypoxia.

Language: English

Document Type: Research article

Affiliations: 1: Institute for Biomedical Aging Research, Austrian Academy of Sciences, Peter-Mayr-Str. 4b, A-6020 Innsbruck, Austria 2: Tyrolean Cancer Research Institute, Innrain 66, A-6020 Innsbruck, Austria 3: Department of Transplant Surgery, D. Swarovski Research Laboratory, University Hospital Innsbruck, Anichstrasse 35, A-6020 Innsbruck

Publication date: 2002-01-01