Breeding bacterial blight-resistant hybrid rice with the cloned bacterial blight resistance gene Xa21
Authors: Zhai W.1; Wang W.1; Zhou Y.2; Li X.1; Zheng X.1; Zhang Q.2; Wang G.1; Zhu L.3
Source: Molecular Breeding, Volume 8, Number 4, 2002 , pp. 285-293(9)
Publisher: Springer
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Abstract:
The cloned bacterial blight (BB) resistance gene Xa21 was transferred into Minghui63, a widely used restorer line of indica hybrid rice in China, through an Agrobacterium-mediated system. Molecular and resistance analyses revealed that the Xa21 gene was integrated in the genomes of transgenic plants and their progeny inherited resistance stably. For the purpose of hybrid breeding, Xa21 transgenic homozygous restorer lines were selected through `within-lane' dosage comparison of hybridization signal in combination with PCR and resistance analyses. The selected transgenic restorer lines were then crossed with a commonly used sterile line, Zhenshan97A, to produce Xa21 transgenic hybrid rice, Shanyou63-Xa21. The hybrid rice plants with Xa21 displayed high broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo) races and maintained elite agronomic characters of Shanyou63. The propagation of this BB-resistant hybrid variety with Xa21 will benefit rice production.
Keywords: blight disease resistance; Indica hybrid rice; Marker-assisted selection; Transgenic restorer line; Xa21
Language: English
Document Type: Regular paper
Affiliations: 1: Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China 2: Institute of Crop Breeding and Cultivation, Chinese Academy of Agricultural Sciences, Beijing 100080, China 3: Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China (fax: 086-10-64873428; e-mail: lhzhu@igtp.ac.cn)
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Click here for Page Help