IngentaConnect Transgenic rice with reduced glutelin content by transformation w...

Authors: Maruta Y.¹; Ueki J.²; Saito H.²; Nitta N.²; Imaseki H.²

Source: Molecular Breeding, Volume 8, Number 4, 2002 , pp. 273-284(12)

Publisher: Springer

Abstract:

Rice plants were genetically transformed with a glutelin A antisense gene construct that was characterized by eight tandem repeats of a ca. 0.3 kb fragment at the 5 prime end of full-length glutelin A cDNA. Two transgenic rice lines, H39-59 and H75-3, homozygous for the glutelin A antisense gene were selected from T_2 plants, and their T_4generation was examined. When grown in an open paddy field, T_4plants of both transgenic lines showed 20% to 40% reduction in the glutelin content of seeds and an increase in the prolamine content. The total seed-protein content of the two transgenic lines was not significantly different from that of the original variety, Tsukinohikari. Northern blot analysis revealed that the steady-state levels of glutelin A transcript also decreased and those of prolamine transcript increased in both H39-59 and H75-3 transgenic lines, and the changes in glutelin mRNA corresponded to changes observed in glutelins during seed development. Rice wine, sake, brewed from grains of the transgenic lines was found to contain 20% to 40% less amino acids and was evaluated as superior in quality to that made from the non-transgenic rice grains.

Keywords: Antisense; Glutelin; Rice; Storage protein; Transgenic plant

Language: English

Document Type: Regular paper

Affiliations: 1: (Yoshiyuki.Maruta@orynova.co.jp) 2: Japan Tobacco Inc., Plant Breeding and Genetics Research Laboratory, 700 Higashibara, Toyoda, Iwata, 438-0802, Japan

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Transgenic rice with reduced glutelin content by transformation with glutelin A antisense gene