Neural cell fate analysis in zebrafish using olig2 BAC transgenics

Authors: Shin J.¹; Park H-C.¹; Topczewska J.M.¹; Mawdsley D.J.¹; Appel B.²

Source: Methods in Cell Science, Volume 25, Numbers 1-2, 2003 , pp. 7-14(8)

Publisher: Springer

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Abstract:

We describe here simple methods for producing transgenic zebrafish reporter lines using BAC clones. The use of BAC clones facilitates creation of useful transgenics as the large amounts of genomic DNA they contain increase the likelihood that reporter gene expression will be properly regulated. Combined with recent advances in live embryo image analysis, this strategy has the potential to greatly advance the investigation of neural cell behavior during development.

Keywords: cerebellum; EGFP; motor neuron; olig2; oligodendrocyte; Purkinje neuron; spinal cord; transgenic; zebrafish

Document Type: Research article

Affiliations: 1: Vanderbilt University, Department of Biological Sciences, Nashville TN 37232, USA 2: Department of Biological Sciences, Vanderbilt University, 2401 Stevenson Center, 1161 21st Avenue South, Nashville TN 37232, USA, Email: b.appel@vanderbilt.edu

Publication date: 2003-01-01