Authors: Díaz-Muñoz, Mauricio; Álvarez-Pérez, Marco; Yáñez, Lucía; Vidrio, Susana; Martínez, Lidia; Rosas, Gisele; Yáñez, Mario; Ramírez, Sotero; Sánchez, Victoria

Source: Molecular and Cellular Biochemistry, Volume 289, Numbers 1-2, September 2006 , pp. 125-136(12)

Publisher: Springer

Abstract:

Myocardial Ca²⁺ overload and oxidative stress are well documented effects associated to isoproterenol (ISO)-induced myocardial necrosis, but information correlating these two issues is scarce. Using an ISO-induced myocardial infarction model, 3 stages of myocardial damage were defined: pre-infarction (0-12 h), infarction (12-24 h) and post-infarction (24-96 h). Alterations in Ca²⁺ homeostasis and oxidative stress were studied in mitochondria, sarcoplasmic reticulum and plasmalemma by measuring the Ca²⁺ content, the activity of Ca²⁺ handling proteins, and by quantifying TBARs, nitric oxide (NO) and oxidative protein damage (changes in carbonyl and thiol groups). Free radicals generated system, antioxidant enzymes and oxidative stress (GSH/GSSG ratio) were also monitored at different times of ISO-induced cardiotoxicity. The Ca²⁺ overload induced by ISO was counterbalanced by a diminution in the ryanodine receptor activity and the Na⁺-Ca⁺² exchanger as well as by the increase in both calcium ATPases activities (vanadate- and thapsigargine-sensitive) and mitochondrial Ca²⁺ uptake during pre-infarction and infarction stages. Pro-oxidative reactions and antioxidant defences during the 3 stages of cardiotoxicity were observed, with maximal oxidative stress during the infarction. Significant correlations were found among pro-oxidative reactions with plasmalemma and sarcoplasmic reticulum Ca²⁺ ATPases, and ryanodine receptor activities at the onset and development of ISO-induced infarction. These findings could be helpful in the design of antioxidant therapies in this pathology.

Keywords: ryanodine receptor; inositol trisphosphate receptor; Ca2+-ATPases; xanthine oxidase; nitric oxide; glutathione

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s11010-006-9155-1

Affiliations: 1: Email: vchagoya@ifc.unam.mx

Publication date: 2006-09-01

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• In this Subject: Anatomy & Physiology ,  Biology ,  Biochemistry
• By this author: Díaz-Muñoz, Mauricio ;  Álvarez-Pérez, Marco ;  Yáñez, Lucía ;  Vidrio, Susana ;  Martínez, Lidia ;  Rosas, Gisele ;  Yáñez, Mario ;  Ramírez, Sotero ;  Sánchez, Victoria

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