Immunomodulatory and cytoprotective role of RP‐1 in ‐irradiated mice
Source: Molecular and Cellular Biochemistry, Volume 254, Numbers 1-2, 200312 , pp. 73-81(9)
Abstract:RP-1 has been reported to provide protection against lethal ‐irradiation in mice. The present study was undertaken to understand its mechanism of action, especially with respect to modulation of radiation‐induced changes in immune cell function, plasma antioxidant potential, cell cycle perturbations, apoptosis in mouse bone marrow cells, and micronuclei frequency in mice reticulocytes. 2 Gy reduced mitogenic response of splenic lymphocytes significantly at 48 h. Pre‐irradiation RP‐1 treatment significantly countered the radiation‐induced loss of splenocyte proliferation. RP‐1 treatment, with or without radiation, suppressed macrophage activation as compared to control. Irradiation decreased plasma antioxidant status significantly (p < 0.05) at 1 and 2 h (4.8 ± 0.224 and 4.9 ± 0.057 mM Fe2+) as compared to control (6.29 ± 0.733 mM Fe2+) that was countered by RP‐1 pre‐treatment significantly (p < 0.05). RP‐1 and irradiation individually caused G2 delay in bone marrow cells. RP‐1 pre‐treatment augmented radiation‐induced G2 delay and elicited significant (p < 0.05) recovery in S phase fraction at 48 h in comparison to irradiated group. Radiation‐induced apoptosis (3%) was significantly higher than the control. RP‐1 pre‐treatment further enhanced apoptosis frequency (7.2%) in bone marrow cells. RP‐1 pre‐treatment significantly (p < 0.05) reduced (1.23%) the radiation‐induced MN frequency (2.9%) observed at 48 h post‐irradiation interval. Since the radioprotective manifestation of RP‐1 is mediated through multiple mechanisms, needs further investigation.
Document Type: Research Article
Affiliations: 1: Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi-110 054, India, Email: firstname.lastname@example.org 2: Department of Radiation Biology, Institute of Nuclear Medicine and Allied Sciences, Brig. SK Mazumdar Marg, Timarpur, Delhi, India 3: Department of Biosciences, Himachal Pradesh University, Shimla, Himachal Pradesh, India
Publication date: 200312