A straight-forward method of optimising protein solubility for NMR

Author: Howe, Peter

Source: Journal of Biomolecular NMR, Volume 30, Number 3, November 2004 , pp. 283-286(4)

Publisher: Springer

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Abstract:

Maximising solubility is a key step in applying solution-state NMR to proteins. The ‘microbatch’ crystallisation screening method can be adapted to screen for protein solubility. In this approach, drops of test solutions are placed under paraffin oil in 96-well screening plates. This requires very small amounts of protein, is easy to set up and is readily automatable.

Keywords: microbatch; protein NMR; protein solubility; structural genomics

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10858-004-3739-4

Affiliations: 1: Analytical Sciences, 011011011011011011011011Syngenta, Jealott’s Hill Research Centre, Bracknell, Berkshire, 011011011011011011011011011RG42 6EY, 011011011011011011011011011U.K, Email: peter.howe@syngenta.com

Publication date: 2004-11-01