Spin-state-selective coherence transfer via intermediate states of two-spin coherence in IS spin systems: Application to E.COSY-type measurement of J coupling constants
Authors: Sørensen M.D.; Meissner A.; Sørensen O.W.
Source: Journal of Biomolecular NMR, Volume 10, Number 2, September 1997 , pp. 181-186(6)
It is demonstrated that a new pulse sequence element, Spin-State-Selective Coherence Transfer (S^3CT), via an intermediate state of heteronuclear IS zero- or double-quantum coherence can transfer the two single-quantum coherences on one of the spins exclusively to any one of the two single-quantum coherences on the other spin. This fact is used for editing into two subspectra that are most suitable for extraction of homo- or heteronuclear J coupling constants when S^3CT is combined with homonuclear coherence transfer during a mixing period. Experimental confirmation is obtained using a ^15N-labeled 58-residue protein, the C-terminal Kunitz domain from human type VI collagen. The J coupling con- stants determined include ^3J_HN-Hα and ^3J_N-Hβ related to the and ^1 angles, respectively.
Document Type: Regular paper
Publication date: 1997-09-01