Spin-state-selective coherence transfer via intermediate states of two-spin coherence in IS spin systems: Application to E.COSY-type measurement of J coupling constants

Authors: Sørensen M.D.; Meissner A.; Sørensen O.W.

Source: Journal of Biomolecular NMR, Volume 10, Number 2, September 1997 , pp. 181-186(6)

Publisher: Springer

Abstract:

It is demonstrated that a new pulse sequence element, Spin-State-Selective Coherence Transfer (S^3CT), via an intermediate state of heteronuclear IS zero- or double-quantum coherence can transfer the two single-quantum coherences on one of the spins exclusively to any one of the two single-quantum coherences on the other spin. This fact is used for editing into two subspectra that are most suitable for extraction of homo- or heteronuclear J coupling constants when S^3CT is combined with homonuclear coherence transfer during a mixing period. Experimental confirmation is obtained using a ^15N-labeled 58-residue protein, the C-terminal Kunitz domain from human type VI collagen. The J coupling con- stants determined include ^3J_HN-Hα and ^3J_N-Hβ related to the and ^1 angles, respectively.

Keywords: E.COSY; Multidimensional NMR; J coupling constants; S3CT

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Chemistry, Carlsberg Laboratory, Gamle Carlsberg Vej 10, DK-2500 Valby, Denmark

Publication date: 1997-09-01

• In this: publication
• By this: publisher
• In this Subject: Anatomy & Physiology ,  Analytical Chemistry ,  Internal Medicine
• By this author: Sørensen M.D. ;  Meissner A. ;  Sørensen O.W.

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