Pulse schemes for the measurement of ^3J_C′C and ^3J_NCscalar couplings in ^15N,^13C uniformly labeled proteins
Authors: Konrat, R.; Muhandiram, D.R.; Farrow, N.A.; Kay, L.E.
Source: Journal of Biomolecular NMR, Volume 9, Number 4, June 1997 , pp. 409-422(14)
Abstract:Pulse sequences are presented for the measurement of ^3J_C′C and ^3J_NC scalar couplings for all C^ containing residues in ^15N,^13C uniformly labeled proteins. The methods described are based on quantitative J correlation spectroscopy pioneered by Bax and co-workers [Bax et al. (1994) Methods Enzymol., 239, 79–105]. The combination of ^3J_C′C and ^3J_NC scalar coupling constants allows the assignment of discrete rotameric states about the _1torsion angle in cases where such states exist or, alternatively, facilitates the establishment of noncanonical _1conformations or the presence of rotameric averaging. The methods are applied to a 1.5 mM sample of staphylococcal nuclease.
Document Type: Regular Paper
Affiliations: Protein Engineering Network of Centres of Excellence and Departments of Medical Genetics, Biochemistry and Chemistry, University of Toronto, 1 Kings College Circle, Toronto, ON, Canada M5S 1A8
Publication date: 1997-06-01