IngentaConnect High frequency plantlet regeneration from cotyledonary node cultu...

Authors: Nayak, Pranati; Behera, P.; Manikkannan, Thirunavoukkarasu¹

Source: In Vitro Cellular and Development Biology - Plant, Volume 43, Number 3, June 2007 , pp. 231-236(6)

Publisher: Springer

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Abstract:

High frequency plantlet regeneration was achieved in cotyledonary nodes of Aegle marmelos. Cotyledonary nodes from 1 mo. old in vitro grown seedlings of A. marmelos were cultured on Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA) (0-8.8 μM), kinetin (KIN) (0-9.4 μM), and indole-3-acetic acid (IAA) (0-1.14 μM) either alone or in combinations. The highest regenerative response was observed on medium containing 6.6 μM BA + 1.14 μM IAA where approximately 86.6% of the cultures responded with an average shoot numbers of 487.5 per explant in 7-wk time. Cultures maintained on KIN-supplemented medium showed very poor response. in vitro responded shoots were transferred to root induction medium consisting of half-strength MS supplemented with auxins IAA, indole-3-butyric acid (IBA), or α-naphthalene acetic acid (NAA). Rooting was best in medium supplemented with 14.7 μM IBA. Rooted plantlets were acclimatized and transferred to the field with 80% survival rate.

Keywords: Auxin; Bael; Cytokinin; MS medium; Rutaceae

Document Type: Research article

DOI: 10.1007/s11627-006-9013-6

Affiliations: 1: Email: mtarasu@yahoo.com

High frequency plantlet regeneration from cotyledonary node cultures of Aegle marmelos (L.) Corr.