Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique

Authors: Marassi, María; Scocchi, Adriana; Gonzalez, Ana

Source: In Vitro Cellular and Development Biology - Plant, Volume 42, Number 1, January 2006 , pp. 31-36(6)

Publisher: Springer

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Abstract:

Anther-derived rice (Oryza sativa L. ssp. japonica variety Yerua P.A.) plants were obtained after cryopreservation by an encapsulation/dehydration technique. Immature anthers, excised from spikelets pretreated at 8°C for 8d, were encapsulated in calcium alginate beads. The beads were cultured on N6 medium with 11.5 μM naphthalenaecetic acid (NAA) and 2.3 μM 6-furfurylaminopurine (KIN). Fifteen percent of the encapsulated anthers formed calluses when pretreated with sucrose for 3 d in liquid medium, desiccated on silica gel, slowly cooled to −30°C, immersed in liquid nitrogen (LN), thawed, and recultured. The cryopreserved encapsulated anthers produced 1.67 shoots/callus, in contrast to the control (non-cooled encapsulated anthers), which produced 6 shoots/callus. Eighty percent of the plantlets developed into normal plants after being transferred to greenhouse conditions. Histological observations showed that the origin of the plants was not modified by the cryopreservation process.

Keywords: Oryza sativa L; cryopreservation; encapsulation/dehydration; androgenesis

Document Type: Research article

DOI: http://dx.doi.org/10.1079/IVP2005746

Affiliations: 1: Email: marassi@agr.unne.edu.ar

Publication date: 2006-01-01