Using Amplified Fragment Length Polymorphisms (AFLP) to Study Genetic Variability in Several Freshwater Rotifer Species

Authors: Hernández-Delgado, Sanjuana¹; Mayek-Pérez, Netzahualcoyotl²; Santos-Medrano, Gustavo³; Rico-Martínez, Roberto⁴

Source: Hydrobiologia, Volume 546, Number 1, September 2005 , pp. 109-115(7)

Publisher: Springer

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Abstract:

We have used amplified fragment length polymorphisms (AFLP) to investigate the potential of this technique as a tool to measure genetic variability in eight species of freshwater rotifers: Brachionus calyciflorus, Lecane bulla, L. luna, L. quadridentata, Plationus patulus, Philodina acuticornis odiosa, Rotaria neptunia, and R. rotatoria. We used nine combinations of oligonucleotides. We observed a total of 806 amplified bands, 798 polymorphic and 8 monomorphic. The data were analyzed using cluster analysis with UPGMA, first within each set of oligonucleotide combination and finally using all nine combinations. Our best dendrogram clearly separated monogononts from digononts, and grouped the species of monogononts in the two genera. However, it grouped R. neptunia with P. acuticornis odiosa rather than with R. rotatoria. These results are discussed in view of recent works in the literature measuring genetic variability and discussing the phylogeny of the Rotifera.

Keywords: DNA; genetic diversity; AFLP; phylogeny; Rotifera

Document Type: Research article

DOI: 10.1007/s10750-005-4106-5

Affiliations: 1: Instituto Tecnológico Agropecuario de Aguascalientes (ITA 20), Km 18 Carretera Aguascalientes-San Luis Potosí, 20330, El Llano, Aguascalientes, México, Email: rrico@correo.uaa.mx 2: Centro de Biotecnología Genómica, Instituto Politécnico Nacional., Blvd. Del Maestro s/n esq. Elías Piña Col. Narciso Mendoza, C.P. 88710, Reynosa, Tamaulipas, México, 3: Departamento de Química, Universidad Autónoma de Aguascalientes Centro Básico, Avenida Universidad 940, C.P. 20100, Ags, Aguascalientes, México, 4: Departamento de Química, Universidad Autónoma de Aguascalientes Centro Básico, Avenida Universidad 940, C.P. 20100, Ags, Aguascalientes, México, Email: rrico@correo.uaa.mx

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