Home >> Genetica, Volume 122, Number 1

Towards a mutant map of the mouse – new models of neurological, behavioural, deafness, bone, renal and blood disorders

Authors: Sohaila Rastan1; Tertius Hough2; Amy Kierman3; Rachel Hardisty2; Alexandra Erven3; Ian C. Gray1; Stepanie Voeling4; Adrian Isaacs5; Hsun Tsai2; Mark Strivens2; Rebecca Washbourne2; Claire Thornton2; Simon Greenaway2; Mazda Hewitt2; Stefan McCormick2; Rachael Selley2; Christine Wells2; Zuzanna Tymowska-Lalanne2; Phil Roby2; Philomena Mburu2; Derek Rogers1; Jim Hagan1; Charlie Reavill1; Kay Davies5; Peter Glenister2; Elizabeth M. C. Fisher6; Josephine Martin7; Lucy Vizor2; Mark Bouzyk1; David Kelsell1; J. -L. Guenet4; Karen P. Steel3; Steve Sheardown1; Nigel Spurr1; Ian Gray1; Jo Peters2; Patrick M. Nolan2; A. Jacqueline Hunter1

Source: Genetica, Volume 122, Number 1, September 2004 , pp. 47-49(3)

Publisher: Springer

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Abstract:

With the completion of the first draft of the human genome sequence, the next major challenge is assigning function to genes. One approach is genome-wide random chemical mutagenesis, followed by screening for mutant phenotypes of interest and subsequent mapping and identification of the mutated genes in question. We (a consortium made up of GlaxoSmithKline, the MRC Mammalian Genetics Unit and Mouse Genome Centre, Harwell, Imperial College, London, and the Royal London Hospital) have used ENU mutagenesis in the mouse for the rapid generation of novel mutant phenotypes for use as animal models of human disease and for gene function assignment (Nolan et al., 2000). As of 2003, 35,000 mice have been produced to date in a genome-wide screen for dominant mutations and screened using a variety of screening protocols. Nearly 200 mutants have been confirmed as heritable and added to the mouse mutant catalogue and, overall, we can extrapolate that we have recovered over 700 mutants from the screening programme. For further information on the project and details of the data, see http://www.mgu.har.mrc.ac.uk/mutabase.

Keywords: ENU mutagenesis; mouse mutants

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10709-004-1930-x

Affiliations: 1: GlaxoSmithKline, 2: MRC Mammalian Genetics Unit and Mouse Genome Centre, 3: MRC Institute of Hearing Research, UK 4: Institut Pasteur, France 5: Department of Human Anatomy and Genetics, University of Oxford, UK 6: Neurogenetics Department, Imperial College, 7: Department of Morbid Anatomy, Queen Mary and Westfield College, UK

Publication date: 2004-09-01

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