The Physical Gene Hsp70 Map on Polytene Chromosomes of Anopheles darlingi from the Brazilian Amazon

Authors: Rafael M.S.¹; Tadei W.P.²; Hunter F.F.³

Source: Genetica, Volume 121, Number 1, May 2004 , pp. 89-94(6)

Publisher: Springer

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Abstract:

In situ hybridization was used to determine the physical location of the Hsp70 genes in salivary polytene chromosomes of Anopheles darlingi from Manaus and Macapá, Brazil, and to assess the usefulness of the Hsp70 locus as a genetic marker in A. darlingi populations. In both populations, the double markings corresponding to the Hsp70-12A and Hsp70-14A genes were located on the right arm of chromosome 2. The Hsp70 locus was considered to be an excellent marker for studying chromosomal evolution and relationships among A. darlingi populations.

Keywords: Anopheles darlingi; Hsp70 genes; In situ hybridization; polytene chromosomes

Document Type: Research article

DOI: http://dx.doi.org/10.1023/B:GENE.0000019959.45267.d7

Affiliations: 1: Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2936, Bairro Petrópolis, 69083-000, Manaus, AM, Brasil (Phone: +55-02192-6433066; msrafael@inpa.gov.br), Fax: +55-02192-6433061, Email: msrafael@inpa.gov.br 2: Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia (INPA), Av. André Araújo, 2936, Bairro Petrópolis, 69083-000, Manaus, AM, Brasil 3: Department of Biological Sciences, Brock University, St. Catharines, Ont., Canada L2S 3A1 (Phone: +1-905-6885550;, Fax: +1-905-6881855)

Publication date: 2004-05-01