Molecular Phylogeny of Genus Vigna Subgenus Ceratotropis Based on rDNA ITS and atpB-rbcL Intergenic Spacer of cpDNA Sequences

Authors: Doi K.¹; Kaga A.²; Tomooka N.²; Vaughan D.A.³

Source: Genetica, Volume 114, Number 2, February 2002 , pp. 129-145(17)

Publisher: Springer

Abstract:

The genetic diversity and phylogenetic relationships among species in the genus Vigna subgenus Ceratotropis were investigated using sequence data from the ribosomal DNA ITS and atpB-rbcL intergenic spacer of chloroplast DNA regions. While both sets of sequences were of similar lengths about 700 bp the rDNA-ITS was more informative than atpB-rbcL having 170% more polymorphic sites and five times as many parsimony-informative sites. The atpB-rbcL spacer may be appropriate for analysis of taxa above the species level in the genus Vigna. Results of analyzing rDNA-ITS revealed, with low level of statistical bias, separation of the subgenus into three groups that correspond to the three sections Aconitifoliae, Angulares, and Ceratotropis. The ancestral section is Aconitifoliae based on comparison with the outgroup species cowpea, Vigna unguiculata. The V. minima complex, V. minima, V. riukiuensis, and V. nakashimae, has a distinct evolutionary path within section Angulares. Other species in section Angulares are very closely related except V. trinervia. Vigna trinervia has an intermediate position between sections. Sequence data suggests one genome donor to V. reflexo-pilosa came from a lineage within section Angulares close to V. exilis, V. hirtella, and V. umbellata. Data presented supports the view that section Angulares is the most recently diversified section in the subgenus, as inferred by short terminal branch lengths among the species of this section.

Keywords: atpB-rbcL spacer; Ceratotropis; Evolution; rDNA-ITS; Vigna

Language: English

Document Type: Regular paper

Affiliations: 1: Japan Science and Technology Corporation, 1-8 Honcho 4-chome, Kawaguchi City, Saitama, 332-0012, Japan 2: Crop Evolutionary Dynamics Laboratory, National Institute of Agrobiological Sciences, Kannondai 2-1-2, Tsukuba, Ibaraki, 305-8602, Japan 3: Author for correspondence (Phone: 81-298-38-7474; Fax: 81-298-38-7408; E-mail: duncan@affrc.go.jp

Publication date: 2002-02-01

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• In this Subject: Pathology ,  Genetics
• By this author: Doi K. ;  Kaga A. ;  Tomooka N. ;  Vaughan D.A.

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