IngentaConnect Single-chain 434 repressors with altered DNA-binding specificitie...

Authors: Simoncsits A.¹; Tjörnhammar M.-L.¹; Wang S.¹; Pongor S.¹

Source: Genetica, Volume 106, Number 1-2, October 1999 , pp. 85-92(8)

Publisher: Springer

Abstract:

Combinatorial mutant libraries of the single-chain 434 repressor were used to discover novel DNA-binding specificities. Members of the library contain one wild type domain and one mutant domain which are connected by a recombinant peptide linker. The mutant domain contains randomized amino acids in place of the DNA-contacting residues. The single-chain derivatives are expected to recognize artificial operators containing the DNA sequence of ACAA – 6 base-pairs – NNNN, where ACAA is bound by the wild-type and NNNN by the mutant domain. An invivo library screening method was used to isolate mutant DNA-binding domains which recognize the TTAA site of an asymmetric operator. Several mutants showed high affinity binding to the selection target and also strong (up to 80 fold) preference for TTAA over the wild type TTGT sequence. Some of the isolated mutants bound with very high affinities (10–50 pM) to operators containing the TTAC sequence, a close homologue of the TTAA selection target.

Keywords: combinatorial library; helix-turn-helix proteins; protein–DNA interaction; selection; single-chain proteins

Language: English

Document Type: Regular paper

Affiliations: 1: International Centre for Genetic Engineering and Biotechnology (ICGEB), Area Science Park, Padriciano 99, I-34012 Trieste, Italy

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Single-chain 434 repressors with altered DNA-binding specificities