Measurement of channel catfish (Ictalurus punctatus) plasma insulin in species-specific radioimmunoassay

Authors: Plisetskaya E.M.^{1, 2, 3}; Bondareva V.M.⁴; Leonard J.B.K.^{5, 6}; Conlon J.M.⁷; Mommsen T.P.⁸; Silverstein J.T.^{5, 9}

Source: Fish Physiology and Biochemistry, Volume 25, Number 1, 2001 , pp. 73-82(10)

Publisher: Springer

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Abstract:

A single antibody, equilibrium, species specific radioimmunoassay (RIA) for catfish insulin was developed and validated for measurements of plasma levels of insulin in juvenile and adult channel catfish. The RIA appeared to be sensitive (20 pg/tube) and stable with intra-assay coefficient of variation between 5.7% and 3.1% and inter-assay coefficient of variation of 6.5%. The blood was collected from two strains of catfish, USDA 103 and Norris, which differ in food consumption and growth rates, the former strain consuming more food and growing faster (Silverstein et al. 1999). The experiments conducted from December to May included fasting and feeding periods from 5 to 14 days as well as either intravenous or intraperitoneal administration of glucagon-like peptide known for its anorexic effect in both mammals and fish (Silverstein et al. 2001). As in other piscine species studied before, fed fish had higher plasma insulin content than food-deprived fish. Feeding status prevailed over other treatments as the primary physiological factor determining circulating plasma insulin levels.

Keywords: catfish insulin; nutritional status; plasma levels; seasonal changes; special-specific radioimmunoassay

Language: English

Document Type: Research article

Affiliations: 1: School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA 98195-5100, USA 2: (Phone: 206 685-3265; Fax: 206 685-3275 3: E-mail: emp@u.washington.edu) 4: Sechenov Institute of Evolutionary Physiology and Biochemistry Russian Academy of Sciences, St. Petersburg 194223, Russia 5: USA/ARS Catfish Genetics Research Unit, Thad Cochran National Warmwater Aquaculture Center, PO Box 38, Stoneville, MS, 38776, USA 6: Present address: Department of Biology, Northern Michigan University, Marquette, MI 49855, USA 7: Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha NE 68178-0405, USA 8: Department of Microbiology and Biochemistry, Petch Building, PO Box 3055, University of Victoria, Victoria, B.C., V8W 3P6 9: Present address: USDA/ARS, National Center for Cool and Cold Water Aquaculture, 11876 Leetown Road, Kearneysville, WV 25430, USA

Publication date: 2001-01-01

Measurement of channel catfish (Ictalurus punctatus) plasma insulin in species-specific radioimmunoassay

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