Structural characterization of the trisaccharide chain from the skin mucus glycoprotein of the rainbow trout, Salmo gairdneri

Authors: Sumi T.^{1, 2, 3}; Hama Y.¹; Nakagawa H.¹; Maruyama D.¹; Asakawa M.⁴

Source: Fish Physiology and Biochemistry, Volume 25, Number 1, 2001 , pp. 11-17(7)

Publisher: Springer

Abstract:

Skin mucus glycoprotein (RGP) was extracted from the skin mucus of rainbow trout, Salmo gairdneri. Alkaline borohydride treatment of RGP yielded a major oligosaccharide alditol NeuAc2 to6GalNAc-ol and several minor oligosaccharide alditols. A trisaccharide alditol (OS-I) was purified by Sephadex G-25 gel filtration and high-performance liquid chromatography (HPLC). The amount of OS-I was estimated to be 7% in RGP from the amount of GalNAc-ol. Structural determination of the OS-I was examined. Structure of OS-I was identified to be NeuAc2 to6 (GalNAc1 to3) GalNAc-ol by -N-acetylgalactosaminidase digestion, methylation analysis, and ¹H-NMR spectroscopy.

Keywords: mucus; mucus glycoprotein; rainbow trout; sialoglycoprotein; trisaccharide

Language: English

Document Type: Research article

Affiliations: 1: Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga 840-8502, Japan 2: (Phone: +81-(0)952-34-4413; Fax: +81-(0)952-34-4412 3: E-mail: sumi@mb.infosaga.or.jp) 4: Laboratory of Food Science, Faculty of Education, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan

Publication date: 2001-01-01

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• In this Subject: Zoology
• By this author: Sumi T. ;  Hama Y. ;  Nakagawa H. ;  Maruyama D. ;  Asakawa M.

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