Structural characterization of the trisaccharide chain from the skin mucus glycoprotein of the rainbow trout, Salmo gairdneri
Authors: Sumi T.1, 2, 3; Hama Y.1; Nakagawa H.1; Maruyama D.1; Asakawa M.4
Source: Fish Physiology and Biochemistry, Volume 25, Number 1, 2001 , pp. 11-17(7)
Publisher: Springer
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Abstract:
Skin mucus glycoprotein (RGP) was extracted from the skin mucus of rainbow trout, Salmo gairdneri. Alkaline borohydride treatment of RGP yielded a major oligosaccharide alditol NeuAc
2 to6GalNAc-ol and several minor oligosaccharide alditols. A trisaccharide alditol (OS-I) was purified by Sephadex G-25 gel filtration and high-performance liquid chromatography (HPLC). The amount of OS-I was estimated to be 7% in RGP from the amount of GalNAc-ol. Structural determination of the OS-I was examined. Structure of OS-I was identified to be NeuAc2 to6 (GalNAc1 to3) GalNAc-ol by -N-acetylgalactosaminidase digestion, methylation analysis, and 1H-NMR spectroscopy.
Keywords: mucus; mucus glycoprotein; rainbow trout; sialoglycoprotein; trisaccharide
Language: English
Document Type: Research article
Affiliations: 1: Department of Applied Biological Sciences, Faculty of Agriculture, Saga University, 1 Honjo-machi, Saga 840-8502, Japan 2: (Phone: +81-(0)952-34-4413; Fax: +81-(0)952-34-4412 3: E-mail: sumi@mb.infosaga.or.jp) 4: Laboratory of Food Science, Faculty of Education, Kumamoto University, Kurokami, Kumamoto 860-8555, Japan
- Free Content
- New Content
- Subscribed Content
- Free Trial Content
Click here for Page Help