Genotypic differences in callus formation and regeneration of somatic embryos in Cyclamen persicum Mill

Authors: Winkelmann, Traud¹; Serek, Margrethe²

Source: Euphytica, Volume 144, Numbers 1-2, July 2005 , pp. 109-117(9)

Publisher: Springer

Buy & download fulltext article:

Price: $47.00 plus tax (Refund Policy)

Abstract:

This study was performed in order to check the applicability of a protocol for somatic embryogenesis in Cyclamen persicum and to compare the in vitro response of cultivars belonging to different cyclamen growth types and flower colours obtained from two breeders. One hundred ovules per plant were prepared from 32 tested F₁ hybrid cultivars represented by five plants each. Callus induction monitored after 8 weeks varied between 42 and 85%. Variation occurred between replications and single plants of a cultivar. Consistency and colour of the developing calluses were diverse. Only in 1 cultivar out of the 32 tested no regeneration of somatic embryos was obtained. The regeneration rates varied from 0 to 65% among cultivars. In the group of midi-type cultivars the regeneration response was lower than that in mini and giganteum types. High regeneration rates were observed in cultivars with flamed flowers.

Keywords: inheritance; regeneration ability; somatic embryogenesis

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10681-005-5038-x

Affiliations: 1: Institute of Floriculture, Tree Nursery Science and Plant Breeding, Section of Floriculture, University of Hannover, Herrenhaeuser Strasse 2, D-30419, Hannover, Germany, Email: traud.winkelmann@zier.uni-hannover.de 2: Institute of Floriculture, Tree Nursery Science and Plant Breeding, Section of Floriculture, University of Hannover, Herrenhaeuser Strasse 2, D-30419, Hannover, Germany,

Publication date: 2005-07-01