Shopping cart
Characterisation of Meloidogyne species from China using Isozyme Phenotypes and Amplified Mitochondrial DNA Restriction Fragment Length Polymorphism
Authors: Xu J.1; Liu P.2; Meng Q.2; Long H.2
Source: European Journal of Plant Pathology, Volume 110, Number 3, March 2004 , pp. 309-315(7)
Publisher: Springer
Abstract:
Forty-six Meloidogyne populations from 14 provinces of China were characterised in terms of malate dehydrogenase, esterase phenotypes and HinfI restriction profiles of amplification products from the mitochondrial DNA (mtDNA) region between the COII and lrRNA genes. Isozyme phenotyping revealed that 29 of the populations were M. incognita, six were M. javanica, six were M. arenaria, three were M. hapla and two were M. enterolobii. HinfI restriction patterns of the COII-lrRNA region correlated with nematode isozyme phenotypes and enabled reliable differentiation and identification of the five root-knot nematodes occurring in China. The size and sequence of the mtDNA amplification product were determined for the first time for M. enteroloii, a potentially economically important crop pathogen. Sequence comparison showed that the sequence of the intergenic region between the COII and lrRNA genes for M. enterolobii was identical to that reported for M. mayaguensis. Together with published observations on morphology, host range and esterase phenotype of the two nominal species, the mtDNA sequence evidence suggests that M. mayaguensis could be conspecific with M. enterolobii.Keywords: esterase; identification; malate dehydrogenase; Meloidogyne enterolobii; mtDNA-PCR–RFLP; root-knot nematodes
Document Type: Research article
DOI: http://dx.doi.org/10.1023/B:EJPP.0000019800.47389.31
Affiliations: 1: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China ), Fax: +86 25 4395246, Email: jianhuaxu@njau.edu.cn 2: Department of Plant Pathology, Nanjing Agricultural University, Nanjing 210095, People's Republic of China
Publication date: 2004-03-01
- In this: publication
- By this: publisher
- In this Subject: Plant Culture , Botany
- By this author: Xu J. ; Liu P. ; Meng Q. ; Long H.
Tools
Receive new issue alert
Latest TOC RSS FeedKey
Print this page