Identification and Characterisation of Bacteria Causing Soft-rot in Agave tequilana

Authors: Jiménez-Hidalgo I.¹; Virgen-Calleros G.²; Mart'inez-de la Vega O.³; Vandemark G.⁴; Olalde-Portugal V.⁵

Source: European Journal of Plant Pathology, Volume 110, Number 3, March 2004 , pp. 317-331(15)

Publisher: Springer

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Abstract:

Agave tequilana is the raw material for the production of the alcoholic beverage tequila. A bacterial disease has affected the A. tequilana crop in recent years. Previous reports based on colony and cell morphology, Gram stain and potato rot indicated that Erwinia sp. is the main pathogen. We isolated a several bacterial isolates capable of producing soft-rot symptoms in greenhouse pathogenicity assays. An extensive characterisation involving pathogenicity tests, fatty acid profile, metabolic and physiological properties, ribosomal DNA sequence and intergenic transcribed spacer amplification (ITS-PCR) and restriction banding pattern (ITS-RFLP) was made of each isolate. Three different species: Erwinia cacticida, Pantoea agglomerans and Pseudomonas sp. were identified. Fatty acid and metabolic profiles gave low similarity values of identification but 16S rDNA sequence, ITS-PCR and ITS-RFLP confirmed the identification of E. cacticida. In the phylogenetic tree, E. cacticida from blue agave was grouped neither with E. cacticida type strains nor with Erwinia carotovora. This is the first report that associates E. cacticida with A. tequilana soft-rot symptoms.

Keywords: Agave tequilana; Erwinia cacticida; Pantoea agglomerans; Pseudomonas sp; soft-rot

Document Type: Research article

DOI: 10.1023/B:EJPP.0000019791.81935.6d

Affiliations: 1: Biotechnology and Biochemistry Department, Centro de Investigación y de Estudios Avanzados – IPN, Unidad Irapuato Km 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, México, CP 36500 2: Centro Universitario de Ciencias Biológicas y Agropecuarias, Carretera a Nogales Km 15.5, Las Agujas-Nextipac, Jalisco, México, CP 45110 3: Genetic Engineering Department, Centro de Investigaci'on y de Estudios Avanzados – IPN, Unidad Irapuato Km 9.6 Libramiento Norte Carretera Irapuato-Le'on, Irapuato, M'exico, CP 36500 4: USDA-ARS, 24106 North Bunn Road, Prosser, WA 99350, USA 5: Biotechnology and Biochemistry Department, Centro de Investigación y de Estudios Avanzados – IPN, Unidad Irapuato Km 9.6 Libramiento Norte Carretera Irapuato-León, Irapuato, México, CP 36500; Author for correspondence 52 462 624 5996; ), Fax: +1, Email: volalde@ira.cinvestav.mx

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