The effect of body temperature on the murine electroretinogram

In this: publication
By this: publisher
In this Subject: Ophthalmology
By this author: Kong J. ;&nbsp; Gouras P.

Authors: Kong J.; Gouras P.

Source: Documenta Ophthalmologica, Volume 106, Number 3, May 2003 , pp. 239-242(4)

Publisher: Springer

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Abstract:

Purpose: To study the effect of body temperature on the murine electroretinogram (ERG). Methods: The corneal ERG elicited by a strobe flash from dark-adapted mice was recorded using a saline wick electrode while measuring rectal temperature continuously. The mouse was placed within a cylindrical coil of tubing through which water circulated from a temperature controlled bath. The body temperature of the mouse was changed stepwise between 30 and 37 °C. Results: ERGs of approximately normal configuration were recorded at body temperature ranging between 30 and 37 °C. The maximum amplitude of the a- and b-waves varied linearly with temperature. The rate of change of b-wave amplitude was about 100 V/degree. At 30 °C, maximum b-wave amplitude was about 400 V; at 37 °C it was about 1000 V. A change in body temperature produced a rapid change in ERG amplitude. Conclusion: The murine ERG is very sensitive to changes in temperature. In order to monitor the ERG accurately over time, continuous recording of body temperature is essential.