Immunolocalization and Expression of Kallistatin and Tissue Kallikrein in Human Inflammatory Bowel Disease

Authors: Stadnicki A.¹; Mazurek U.²; Gonciarz M.¹; Plewka D.³; Nowaczyk G.³; Orchel J.⁴; Pastucha E.¹; Plewka A.³; Wilczok T.⁴; Colman R.W.⁵

Source: Digestive Diseases and Sciences, Volume 48, Number 3, March 2003 , pp. 615-623(9)

Publisher: Springer

Abstract:

The distribution of tissue kallikrein (TK) and its plasma inhibitor, kallistatin in plasma and intestinal tissue, was studied in patients with active ulcerative colitis (UC) and Crohn's disease (CD). TK was localized to goblet cells and kallistatin to epithelial cells of normal human intestine. Both proteins are visualized in macrophages inside granulomas in CD as well as in plasmocytes in both CD and UC. Intestinal tissue kallikrein (ITK) and kallistatin are significantly decreased in inflamed intestine compared to noninflammatory controls. TK mRNA is significantly decreased in intestinal biopsy samples from active UC patients compared with inactive patients or controls. Immunoreactive TK is present in plasma in very low concentrations in patients and did not differ in normal subjects. Plasma kallistatin was significantly decreased in patients with active disease compared to normal controls. Our data suggest that release of TK during inflammation plays a role in inflammatory bowel disease.

Keywords: Crohn's disease; ulcerative colitis; bradykinin; goblet cells

Language: English

Document Type: Research article

Affiliations: 1: Department of Internal Medicine, Medical University of Silesia, Katowice, Poland 2: Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Katowice, Poland. Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland 3: Department of Histology and Embryology, Medical University of Silesia, Katowice, Poland 4: Department of Molecular Biology, Biochemistry and Biopharmacy, Medical University of Silesia, Katowice, Poland 5: The Sol Sherry Thrombosis Research Center, Temple University School of Medicine, Philadelphia, Pennsylvania 19140, USA

Publication date: 2003-03-01

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• In this Subject: Gastroenterology
• By this author: Stadnicki A. ;  Mazurek U. ;  Gonciarz M. ;  Plewka D. ;  Nowaczyk G. ;  Orchel J. ;  Pastucha E. ;  Plewka A. ;  Wilczok T. ;  Colman R.W.

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