EGF and TGF-
1 Gene Expression in Chronically Rejecting Small Bowel Transplants
Authors: Kouwenhoven E.A.; Stein-Oakley A.N.; Jablonski P.; De Bruin R.W.F.; Thomson N.M.
Source: Digestive Diseases and Sciences, Volume 44, Number 6, 1 June 1999 , pp. 1117-1123(7)
Publisher: Springer
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Abstract:
Long-term survival of small bowel transplants is hampered by chronic rejection. Epidermal growth factor (EGF) and transforming growth factor (TGF-) have opposing, regulatory roles in normal intestinal physiology and may be involved in the pathogenesis of chronic intestinal rejection. Our aim was to investigate the expression of EGF and TGF-_1 in chronically rejecting small bowel transplants. Orthotopic small bowel transplantation was performed in the allogeneic DA-to-AS rat combination; Cyclosporin was administered temporarily to prevent acute rejection. Controls were DA isografts and normal DA rats. PreproEGF and TGF-_1 gene expression was evaluated by northern blot analysis of the ileum RNA and standardized against glyceraldehyde-3-phosphate-dehydrogenase expression. Allografts demonstrated functional impairment and histological features of chronic rejection, whereas isografts appeared normal. Allografts demonstrated a significant reduction of EGF mRNA when compared to DA isografts. No significant changes were detected in TGF-_1expression in either allogeneic or syngeneic grafts. In conclusion, this study demonstrates reduced preproEGF and preserved TGF-_1 gene expression in chronically rejecting small bowel transplants.
Keywords:
EPIDERMAL GROWTH FACTOR;
TRANSFORMING GROWTHFACTOR-;
CHRONIC REJECTION;
SMALL BOWEL;
TRANSPLANTATION
Language: English
Document Type: Regular paper
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