Evidence for a Nonneural Electrogenic Effect of Cholera Toxin on Human Isolated Ileal Mucosa

Authors: Burleigh D.E.; Borman R.A.

Source: Digestive Diseases and Sciences, Volume 42, Number 9, 1 September 1997 , pp. 1964-1968(5)

Publisher: Springer

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Abstract:

Cholera toxin-induced intestinal secretion in intact rats requires a functioning myenteric plexus. The aim of this investigation was to determine whether neural elements were essential for cholera toxin to produce a secretory effect in human isolated ileum. Mucosal preparations were mounted in Ussing chambers. Cholera toxin was applied apically and short-circuit current monitored for 3 hr, at which point forskolin was given. Cholera toxin (10 g/ml) induced a tetrodotoxin-insensitive increase in short-circuit current in muscle-stripped preparations of human ileum. The increase was not additive with the action of forskolin (25 M). Cholera toxin exerts a marked nonneural secretory effect in human ileal mucosa in vitro , probably by the same mechanism as forskolin, namely elevation of cyclic AMP.