Meiotic Chromosomes as Templates for Microdissection

Authors: Christian A.T.¹; Pattee M.S.²; Marchetti F.²

Source: Chromosome Research, Volume 10, Number 1, 2002 , pp. 45-48(4)

Publisher: Springer

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Abstract:

As templates for chromosome microdissection, meiotic cells offer several advantages over mitotic cells. The pairing of homologous chromosomes at the metaphse plate of the first meiotic division allows the simultaneous isolation of two copies of the same chromosome, and the sex chromosomes are easy to identify in male meiotic cells. We report on a method for making fluorescence in-situ hybridization (FISH) probes from dissected meiotic chromosomes.

Keywords: chromosome; FISH; meiosis; microdissection; mouse

Language: English

Document Type: Regular paper

Affiliations: 1: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, P.O. Box 808, L-452. Livermore, CA, 94551, USA; Tel: (925) 424-5909; Fax: (925) 424-3130; E-mail: christian4@llnl.gov 2: Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory. P.O. Box 808, L-448. Livermore, CA, 94551

Publication date: 2002-01-01