Involvement of Glial Cells in Cyclosporine-Increased Permeability of Brain Endothelial Cells

Authors: Dohgu S.¹; Kataoka Y.²; Ikesue H.¹; Naito M.³; Tsuruo T.³; Oishi R.¹; Sawada Y.⁴

Source: Cellular and Molecular Neurobiology, Volume 20, Number 6, December 2000 , pp. 781-786(6)

Publisher: Springer

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Abstract:

SUMMARY

1. To test whether astrocytes participate in cyclosporine-induced dysfunction of the blood-brain barrier, we examined the effects of cyclosporine on the permeability of the mouse brain endothelial (MBEC4) cells cocultured with C6 glioma cells, each cell layer placed on the top and bottom of the insert membrane, respectively.

2. The presence of C6 cells remarkably aggravated cyclosporine-increased permeability of MBEC4 cells to sodium fluorescein.

3. In light of these findings, the possibility that astroglial cells could contribute to the occurrence of cyclosporine-induced dysfunction of the blood-brain barrier triggering neurotoxicity should be considered.

Keywords: cyclosporine; neurotoxicity; glia@keyword = blood–brain barrier

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan 2: Department of Hospital Pharmacy, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan ykataoka@st.hosp.kyushu-u.ac.jp 3: Institute of Molecular and Cellular Biosciences, University of Tokyo, Bunkyo-ku, Tokyo, 113-0032, Japan 4: Department of Clinical Pharmacy, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan

Publication date: 2000-12-01