Isolation and Microsequencing of a Novel Cotinine Receptor

Authors: Riah O.¹; Dousset J-C.²; Bofill-Cardona E.³; Courrière P.²

Source: Cellular and Molecular Neurobiology, Volume 20, Number 6, December 2000 , pp. 653-664(12)

Publisher: Springer

Abstract:

SUMMARY

1. Nicotine and its main derivative, cotinine, are reported to have distinct central activities in mammals. In this study, the cotinine receptor was separated by biochemical procedures including radio receptor, affinity-chromatography, SDS–PAGE, and N-terminal sequencing assays.

2. Consistently, the results showed that distinctive cotinine receptors exist in different tissues of mammals. In rat brain, the affinity chromatography and [^125I]cotinine receptor essays were used to isolate a 40-kDa protein (p40) with higher affinity for cotinine than alpha-bungarotoxin and nicotine. The N-terminus amino acid sequences of the p40 and its internal tryptic peptides showed no identity to recently described protein sequences, with the exception of homology to the human p205 synovial fluid protein.

3. These results, in agreement with other behavioral studies, are the first molecular evidence for distinctive nicotine and cotinine receptors in mammals.

Keywords: [I]cotinine; nicotine; cotinine; pharmacology; receptor; affinity chromatography; microsequencing

Language: English

Document Type: Regular paper

Affiliations: 1: Laboratoire des Mécanismes d'Action des Nicotianées, Faculté des Sciences Pharmaceutiques, 35, Chemin des Maraichers, 31062 Toulouse Cedex, France. Laboratoire de Biochimie, Département de Biologie, Faculté des Sciences, BP 32/S, 7000, Agadir, Maroc riah@cict.fr 2: Laboratoire des Mécanismes d'Action des Nicotianées, Faculté des Sciences Pharmaceutiques, 35, Chemin des Maraichers, 31062 Toulouse Cedex, France 3: Laboratoire des Mécanismes d'Action des Nicotianées, Faculté des Sciences Pharmaceutiques, 35, Chemin des Maraichers, 31062 Toulouse Cedex, France. Present address: Institute of Pharmacology, Vienna University, A 1090, Vienna, Austria

Publication date: 2000-12-01

• In this: publication
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• In this Subject: Anatomy & Physiology ,  Zoology ,  Neurology & Psychiatry
• By this author: Riah O. ;  Dousset J-C. ;  Bofill-Cardona E. ;  Courrière P.

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