Authors: Wainwright, Cherry; Kang, Liping; Ross, Sean

Source: Cardiovascular Drugs and Therapy, Volume 11, Number 5, November 1997 , pp. 669-678(10)

Publisher: Springer

Abstract:

Previous studies in pigs have shown that the A₁-adenosine receptor agonist, R-PIA, is a potent antifibrillatory agent during myocardial ischaemia and that this effect can be overriden by atrial pacing. However, reports of A₁ -adenosine receptor down-regulation following chronic exposure to A₁ -receptor agonists suggest that this may limit their use as potential antiarrhythmic therapy. The acute and chronic effects of R-PIA were examined on ventricular arrhythmias and hemodynamics in Langendorff-perfused rat isolated hearts subjected to acute regional myocardial ischemia in an attempt to confirm the heart rate dependency of the antifibrillatory mechanism of R-PIA and to assess the effects of chronic treatment on this protection. Acute challenge with R-PIA (10^-10 to 5 × 10^-8 M; n = 10 for all groups) produced a concentration-dependent bradycardia prior to coronary occlusion. Coronary artery occlusion in control hearts (n= 20) resulted in an immediate increase in perfusion pressure, from61 ± 6 to 87 ± 7 mmHg within 5 minutes, followed by a gradual continued rise reaching a maximum of 123 ± 9 mmHg by the end of the 30-minute experimental period. R-PIA significantly attenuated the sustained increase in perfusion pressure in a non-concentration-dependent manner. A concentration of 10^-10 M R-PIA had no effect on the incidence of ventricular fibrillation (VF), while all higher concentrations reduced the incidence of VF to a similar degree (from 60% in controls to10%, 20%, 10%, and 0% with 10^-9, 5× 10^-9, 10^-88, and 5 × 10^-8 M R-PIA, respectively). R-PIA also reduced the total ventricular premature beat (VPB) count, but in a concentration-dependent manner. Chronic treatment of the rats with R-PIA (50 µg/kg ip, bd; n = 10) for 7 days caused a significant attenuation of the bradycardic response to acute perfusion in vitro with R-PIA (10^-8 M) and abolished the attenuation of the sustained rise in perfusion pressure during myocardial ischemia. The antifibrillatory effect of R-PIA, however, was unaffected by chronic pretreatment (VF incidence 0% vs. 70% in control hearts from rats that had been given chronic saline ip injections n = 10; P < 0.01). These results suggest that the bradycardia induced by acute R-PIA may not be the mechanism underlying the antifibrillatory effect of R-PIA, while the reduction in the less severe arrhythmias is heart rate dependent. Furthermore, while chronic treatment with R-PIA significantly attenuates the heart rate response to acute R-PIA challenge, the antifibrillatory properties remain intact

Keywords: A1 -adenosine receptor agonist; R-PIA; ischemic arrhythmias; rat isolated hearts

Document Type: Research article

DOI: http://dx.doi.org/10.1023/A:1007739025010

Publication date: 1997-11-01

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• In this Subject: Cardiovascular Medicine ,  Pathology ,  Pharmacology
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