Studies on the Mechanism Underlying the Antifibrillatory Effect of the A1-Adenosine Agonist, R-PIA, in Rat Isolated Hearts
Authors: Wainwright, Cherry; Kang, Liping; Ross, Sean
Source: Cardiovascular Drugs and Therapy, Volume 11, Number 5, November 1997 , pp. 669-678(10)
Publisher: Springer
Abstract:
Previous studies in pigs have shown that the A1-adenosine receptor agonist, R-PIA, is a potent antifibrillatory agent during myocardial ischaemia and that this effect can be overriden by atrial pacing. However, reports of A1 -adenosine receptor down-regulation following chronic exposure to A1 -receptor agonists suggest that this may limit their use as potential antiarrhythmic therapy. The acute and chronic effects of R-PIA were examined on ventricular arrhythmias and hemodynamics in Langendorff-perfused rat isolated hearts subjected to acute regional myocardial ischemia in an attempt to confirm the heart rate dependency of the antifibrillatory mechanism of R-PIA and to assess the effects of chronic treatment on this protection. Acute challenge with R-PIA (10-10 to 5 × 10-8 M; n = 10 for all groups) produced a concentration-dependent bradycardia prior to coronary occlusion. Coronary artery occlusion in control hearts (n= 20) resulted in an immediate increase in perfusion pressure, from61 ± 6 to 87 ± 7 mmHg within 5 minutes, followed by a gradual continued rise reaching a maximum of 123 ± 9 mmHg by the end of the 30-minute experimental period. R-PIA significantly attenuated the sustained increase in perfusion pressure in a non-concentration-dependent manner. A concentration of 10-10 M R-PIA had no effect on the incidence of ventricular fibrillation (VF), while all higher concentrations reduced the incidence of VF to a similar degree (from 60% in controls to10%, 20%, 10%, and 0% with 10-9, 5× 10-9, 10-88, and 5 × 10-8 M R-PIA, respectively). R-PIA also reduced the total ventricular premature beat (VPB) count, but in a concentration-dependent manner. Chronic treatment of the rats with R-PIA (50 µg/kg ip, bd; n = 10) for 7 days caused a significant attenuation of the bradycardic response to acute perfusion in vitro with R-PIA (10-8 M) and abolished the attenuation of the sustained rise in perfusion pressure during myocardial ischemia. The antifibrillatory effect of R-PIA, however, was unaffected by chronic pretreatment (VF incidence 0% vs. 70% in control hearts from rats that had been given chronic saline ip injections n = 10; P < 0.01). These results suggest that the bradycardia induced by acute R-PIA may not be the mechanism underlying the antifibrillatory effect of R-PIA, while the reduction in the less severe arrhythmias is heart rate dependent. Furthermore, while chronic treatment with R-PIA significantly attenuates the heart rate response to acute R-PIA challenge, the antifibrillatory properties remain intactKeywords: A1 -adenosine receptor agonist; R-PIA; ischemic arrhythmias; rat isolated hearts
Document Type: Research article
DOI: http://dx.doi.org/10.1023/A:1007739025010
Publication date: 1997-11-01
- In this: publication
- By this: publisher
- In this Subject: Cardiovascular Medicine , Pathology , Pharmacology
- By this author: Wainwright, Cherry ; Kang, Liping ; Ross, Sean

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