IngentaConnect Evaluation of the Paratrend Multi-Analyte Sensor for Potential Ut...

Abstract:

Background: Compact and automated sensors are desired for assessing the health of cell cultures in biotechnology experiments. While several single-analyte sensors exist to measure culture health, a multi-analyte sensor would simplify the cell culture system. One such multi-analyte sensor, the Paratrend 7 manufactured by Diametrics Medical, consists of three optical fibers for measuring pH, dissolved carbon dioxide (pCO₂), dissolved oxygen (pO₂), and a thermocouple to measure temperature. The sensor bundle was designed for intra-vascular measurements in clinical settings, and can be used in bioreactors operated both on the ground and in NASA’s Space Shuttle and International Space Station (ISS) experiments. Methods: A Paratrend 7 sensor was placed at the outlet of a bioreactor inoculated with BHK-21 (baby hamster kidney) cells. The pH, pCO₂, pO₂, and temperature data were transferred continuously to an external computer. Cell culture medium, manually extracted from the bioreactor through a sampling port, was also assayed using a bench top blood gas analyzer (BGA). Results: Two Paratrend 7 sensors were used over a single cell culture experiment (64 days). When compared to the manually obtained BGA samples, the sensor had good agreement for pH, pCO₂, and pO₂ with bias (and precision) 0.005(0.024), 8.0 mmHg (4.4 mmHg), and 11 mmHg (17 mmHg), respectively for the first two sensors. A third Paratrend sensor (operated for 141 days) had similar agreement (0.02±0.15 for pH, -4±8 mm Hg for pCO₂, and 24±18 mm Hg for pO₂). Conclusion: The resulting biases and precisions are com- parable to Paratrend sensor clinical results. Although the pO₂ differences may be acceptable for clinically relevant measurement ranges, the O₂ sensor in this bundle may not be reliable enough for the ranges of pO₂ in these cell culture studies without periodic calibration.

Keywords: fiber-optic sensors; mammalian cell culture; pH sensor; pO2 sensor; blood gas analysis

Document Type: Research article

DOI: 10.1023/B:BMMD.0000042054.02940.b6

Affiliations: 1: Biotechnology Program, Wyle Laboratories, Houston, TX 2: Biological Systems Office, NASA, Johnson Space Center, Houston, TX antony.s.jeevarajan@nasa.gov, Email: antony.s.jeevarajan@nasa.gov

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Evaluation of the Paratrend Multi-Analyte Sensor for Potential Utilization in Long-Duration Automated Cell Culture Monitoring