Poly(dimethylsiloxane) (PDMS) and Silicon Hybrid Biochip for Bacterial Culture
Source: Biomedical Microdevices, Volume 5, Number 4, 200312 , pp. 281-290(10)
Abstract:In this study, a novel PDMS/silicon hybrid microfluidic biochip was fabricated and tested for the long-term batch culture of bacterial cells. The PDMS (poly(dimethylsiloxane)) cover with 3-dimensional micro-channels for flow was fabricated using Teflon tubing and hole-punch techniques, without photolithographic methods. The PDMS/silicon hybrid biochip was prepared by bonding of PDMS cover and a silicon chip that had electrodes and micro-fluidic channels defined. The absorption of liquid into PDMS cover was characterized and conditions to prevent drying of nutrient media within the micro-chamber were shown. The absorption of liquid from micro-chambers into the PDMS cover was reduced up to 2.5 times by changing the mixing ratio of PDMS and curing agent from 10 : 1 to 2.5 : 1. In addition, pre-saturation of the PDMS cover with media prior to the incubation resulted in the preservation of liquid in the micro-chambers for up to 22 hours. Optimization of the mixing ratio and pre-saturation of the PDMS cover reduced the drying time 10 times when compared to the unsaturated PDMS cover composed of 10 : 1 ratio of PDMS and curing agent. Listeria innocua and a strain of Escherichia coli, expressing green fluorescent protein (GFP), were successfully cultured in batch mode within the PDMS/silicon hybrid biochip.
Document Type: Research Article
Affiliations: 1: Laboratory of Integrated Biomedical Micro/Nanotechnology and Applications, Birck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical Engineering 2: Department of Biomedical Engineering and the Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907 3: Laboratory of Integrated Biomedical Micro/Nanotechnology and Applications, Birck Nanotechnology Center, School of Electrical and Computer Engineering and the Department of Biomedical Engineering, Email: firstname.lastname@example.org
Publication date: January 1, 2003