Enzymatic Microreactor Using Si, Glass and EPON SU-8

Authors: Strike, D.J.¹; Fiaccabrino G.-C.,¹; Koudelka-Hep, M.¹; de Rooij, N.F.¹

Source: Biomedical Microdevices, Volume 2, Number 3, 1 June 2000 , pp. 175-178(4)

Publisher: Springer

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Abstract:

This article describes the design, fabrication and initial testing of an enzymatic microreactor using bi Si, glass, epoxy and Perspex components. The microreactor geometry was defined by a photostructured EPON SU-8 spacer. This gave two chambers, an upstream chamber (vol. ∼2.7 μl) which was packed with control pore glass (CPG) and a downstream chamber (vol. ∼1.5 μl) which contained the detectors. Eleven EPON SU-8 columns separated the chambers and prevented the CPG from passing. Glucose detection via glucose oxidase immobilized to the CPG was used as a model system. Conversion efficiencies between 15 and 60% at flow rates of 150 and 26 μ1 min^-1 respectively were obtained. Overall the system was found to be robust and to have a lifetime of at least one month.

Keywords: microreactor; microfluidics; EPON SU-8; glucose; control pore glass

Document Type: Regular paper

Affiliations: 1: Institute of Microtechnology, University of Neuchatel, Rue Jaquet-Droz 1 PO Box 3, 2007 Neuchatel, Switzerland

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