Abstract:

Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.

Keywords: protein separation; aqueous two-phase system; affinity partitioning; lysozyme

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Food and Biotechnology, Kyonggi University, Suwon 442–760, Korea 2: Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, 22100 Lund, Sweden