Development of a shuttle vector for screening of strong promoter sequences

Authors: Tangney M.¹; Mitchell W.J.¹; Dolberg-Rasmussen M.²

Source: Biotechnology Techniques, Volume 13, Number 2, February 1999 , pp. 141-144(4)

Publisher: Springer

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Abstract:

A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

Keywords: promoter probe vector; gene expression; transcriptional terminator

Language: English

Document Type: Regular paper

Affiliations: 1: Biological Sciences Department, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland 2: Department of Bacterial Gene Technology, Novo Nordisk A/S, Novo Alle, DK-2880 Bagsværd, Denmark

Publication date: 1999-02-01