Development of a shuttle vector for screening of strong promoter sequences

Authors: Tangney M.1; Mitchell W.J.1; Dolberg-Rasmussen M.2

Source: Biotechnology Techniques, Volume 13, Number 2, February 1999 , pp. 141-144(4)

Publisher: Springer

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

A new promoter probe plasmid, pMOL618, has been specifically designed to be selective for strong promoter sequences. The plasmid contains two origins of replication which allow it to replicate both in Bacillus subtilis and Escherichia coli, as well as an indicator gene which also functions in both backgrounds. The plasmid is therefore useful in the screening of promoter sequences in both organisms. The stringency of the promoter selection is demonstrated using a known strong promoter.

Keywords: promoter probe vector; gene expression; transcriptional terminator

Language: English

Document Type: Regular paper

Affiliations: 1: Biological Sciences Department, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland 2: Department of Bacterial Gene Technology, Novo Nordisk A/S, Novo Alle, DK-2880 Bagsværd, Denmark

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$47.00 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A