A reliable method for precise initial rate determination of enzyme activity under high hydrostatic pressure with simple apparatus - Application to Aspergillus niger fructosyl-transferase activity measurements

Authors: Vannieuwenburgh C.¹; Guibert A.¹; Combes D.²

Source: Biotechnology Techniques, Volume 12, Number 12, December 1998 , pp. 931-934(4)

Publisher: Springer

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Abstract:

An original procedure for initial rate accurate determination of enzyme activity under high hydrostatic pressure is reported. This method, adapted to most kinds of enzyme systems, is based on the use of the linear property of product formation during the initial phase of a reaction and does not require specific high pressure equipment. The reliability of the method was tested and illustrated with the study of Aspergillus niger fructosyl-transferase activity as a function of substrate concentration above 300 MPa. © Rapid Science Ltd. 1998

Keywords: Hydrostatic pressure; Aspergillus niger fructosyl-transferase

Language: English

Document Type: Regular paper

Affiliations: 1: Béghin-Say, Laboratoire de rechercheToulouseFrance 2: Institut national des sciences appliquées, Centre de bioingénierie Gilbert Durand, UMR CNRS 5504, L.A. INRA, Complexe scientifique de Rangueil Toulouse France

Publication date: 1998-12-01