Determination of citrate by FIA using immobilized Enterobacter aerogenes cells and enzyme thermistor/flow microcalorimeter detection

Authors: Svitel J.¹; Vostiar I.¹; Gemeiner P.²; Danielsson B.³

Source: Biotechnology Techniques, Volume 11, Number 12, 1997 , pp. 917-919(3)

Publisher: Springer

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Abstract:

Immobilized whole cells of Enterobacter aerogenes were used as citrate-sensing biocatalyst. The cells efficiently metabolized citrate and the heat released enabled thermometric flow injection analysis (FIA) of citrate with a linear detection range between 0.2 and 6 mM. The immobilized cells were tested for operational stability over 200 runs. Some possible solutions to the problem of dealing with saccharidic substrates, such as glucose, fructose, and sucrose, which may be present with the citrate and interfere with the determination, are also discussed.

Language: English

Document Type: Regular paper

Affiliations: 1: Department of Biotechnology, Technical University, Radlinskho 9 SK-812 37 Bratislava Slovakia 2: Institute of Chemistry, Slovak Academy of Sciences, Dbravsk cesta 9 SK-842 38 Bratislava Slovakia 3: Pure and Applied Biochemistry, Center for Chemistry, Lund University, PO Box 124 S-221 00 Lund Sweden

Publication date: 1997-01-01