On-line monitoring of mammalian cell and yeast fermentations with a commercial biochemical analyzer

Authors: Vriezen N.1; van Dijken J.P.1

Source: Biotechnology Techniques, Volume 11, Number 6, 1997 , pp. 427-430(4)

Publisher: Springer

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Abstract:

A commercial analyzer was tested for on-line monitoring of fermentations. A new sample block was constructed to effectively degas the fermentation broth. The robust analyzer accurately measured glucose up to 110 mmol/l and lactate up to 21 mmol/l at a frequency of 1 measurement per 2 minutes directly in suspensions of mammalian and yeast cells.

Language: English

Document Type: Regular paper

Affiliations: 1: Kluyver Laboratory for Biotechnology, Technical University Delft Julianalaan 67 2628BC Delft the Netherlands

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