Isolation, purification and preliminary characterization of cryophilic proteases of marine origin

Authors: Salamanca, M.H.; Barría, C.; Asenjo, J.A.; Andrews, B.A.

Source: Bioseparation, Volume 10, Numbers 4-5, May 2001 , pp. 237-241(5)

Publisher: Springer

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Abstract:

The isolation and preliminary characterization of a trypsin-like protease with high activity at 20 °C is described. This protease was isolated from Antarctic krill (Euphasia superba) by a two-step chromatography process and the use of zymogram analyses. The protease has a molecular weight of 30 kDa and a pI of 4.1. Its specific activity at 20 °C on BAPNA is 0.5 U/mg.

Keywords: trypsin-like protease; cryophilic; krill

Document Type: Research article

DOI: http://dx.doi.org/10.1023/A:1016383212244

Publication date: 2001-05-01