In vitro regeneration of Solanum nigrum with enhanced solasodine production

Authors: Bhat, M.1; Mujib, A.2; Junaid, A.1; Mahmooduzzafar

Source: Biologia Plantarum, Volume 54, Number 4, December 2010 , pp. 757-760(4)

Publisher: Springer

Buy & download fulltext article:


Price: $47.00 plus tax (Refund Policy)


High frequency of shoot formation was achieved from Solanum nigrum L. leaves on Murashige and Skoog (MS) medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg dm−3 benzylaminopurine and 1.5 mg dm−3 kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first successful induction of in vitro flowering in S. nigrum. The leaf derived shoots were excised and treated with various root promoting PGRs and 0.25 mg dm−3 indole-3-butyric acid produced maximum number of roots (15.2 per plant). Plants were later transplanted in field with 100 % survival. Solasodine content was higher in in vitro raised shoots and leaf derived callus, compared to ex vitro grown shoots.

Keywords: HPTLC; black nightshade; in vitro flowering; plant growth regulators

Document Type: Research Article


Affiliations: 1: Department of Botany, Faculty of Science, Hamdard University, New Delhi, 110062, India 2: Department of Botany, Faculty of Science, Hamdard University, New Delhi, 110062, India, Email:

Publication date: December 1, 2010

Related content


Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content

Text size:

A | A | A | A
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages. print icon Print this page