IngentaConnect Copper-induced oxidative stress and antioxidant defence in Arabid...

Authors: Draob zdot kiewicz M.¹; Skórzy nacute ska-Polit E.²; Krupa Z.²

Source: BioMetals, Volume 17, Number 4, August 2004 , pp. 379-387(9)

Publisher: Springer

Abstract:

Content of reactive oxygen species (ROS): O₂sout^•, H₂O₂ and OH^• as well as activities of antioxidant enzymes: superoxide dismutase (SOD), guaiacol peroxidase (POX) and catalase (CAT) were studied in leaves of Arabidopsis thaliana ecotype Columbia, treated with Cu excess (0, 5, 25, 30, 50, 75, 100, 150 and 300 mgr

M). After 7 days of Cu action ROS content and the activity of SOD and POX increased, while CAT activity decreased in comparison with control. Activities of SOD, POX and CAT were correlated both with Cu concentration (0–75 mgr

M) in the growth medium and with OH^• content in leaves. Close correlation was also found between OH^• content and Cu concentration. Oxidative stress in A. thaliana under Cu treatment expressed in elevated content of O₂sout^•, H₂O₂ and OH^• in leaves. To overcome it very active the dismutase- and peroxidase-related (and not catalase-related, as in other plants) ROS scavenging system operated in A. thaliana. Visual symptoms of phytotoxicity: chlorosis, necrosis and violet colouring of leaves as well as a reduction of shoot biomass occurred in plants.

Keywords: antioxidant enzymes; heavy metal; reactive oxygen species

Document Type: Research article

DOI: 10.1023/B:BIOM.0000029417.18154.22

Affiliations: 1: Department of Plant Physiology, Maria Curie-Sk lstrok odowska University, Akademicka 19, 20-033 Lublin, Poland 37 59 01 mdrazki@biotop.umcs.lublin.pl, Fax: (48-81), Email: mdrazki@biotop.umcs.lublin.pl odowska University, Akademicka 19, 20-033 Lublin, Poland 37 59 01 mdrazki@biotop.umcs.lublin.pl, Fax: (48-81), Email: mdrazki@biotop.umcs.lublin.pl "> 2: Department of Plant Physiology, Maria Curie-Sk lstrok odowska University, Akademicka 19, 20-033 Lublin, Polandodowska University, Akademicka 19, 20-033 Lublin, Poland">

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Copper-induced oxidative stress and antioxidant defence in Arabidopsis thaliana