Large-scale cultivation of adventitious roots of Echinacea purpurea in airlift bioreactors for the production of chichoric acid, chlorogenic acid and caftaric acid

Authors: Wu, Chun-Hua; Murthy, Hosakatte; Hahn, Eun-Joo; Paek, Kee-Yoeup

Source: Biotechnology Letters, Volume 29, Number 8, August 2007 , pp. 1179-1182(4)

Publisher: Springer

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Abstract:

Adventitious roots of Echinacea purpurea were cultured in airlift bioreactors (20 l, 500 l balloon-type, bubble bioreactors and 1,000 l drum-type bubble bioreactor) using Murashige and Skoog (MS) medium with 2 mg indole butyric acid l⁻¹ and 50 g sucrose l⁻¹ for the production of chichoric acid, chlorogenic acid and caftaric acid. In the 20 l bioreactor (containing 14 l MS medium) a maximum yield of 11 g dry biomass l⁻¹ was achieved after 60 days. However, the amount of total phenolics (57 mg g⁻¹ DW), flavonoids (34 mg g⁻¹ DW) and caffeic acid derivatives (38 mg g⁻¹ DW) were highest after 50 days. Based on these studies, pilot-scale cultures were established and 3.6 kg and 5.1 kg dry biomass were achieved in the 500 l and 1,000 l bioreactors, respectively. The accumulation of 5 mg chlorogenic acid g⁻¹ DW, 22 mg chichoric acid g⁻¹ DW and 4 mg caftaric acids g⁻¹ DW were achieved with adventitious roots grown in 1,000 l bioreactors.

Keywords: Adventitious root cultures; Airlift bioreactors; Caffeic acid derivatives; Echinacea purpurea; Large-scale cultures

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10529-007-9399-1

Affiliations: 1: Email: paekky@chungbuk.ac.kr

Publication date: 2007-08-01