Immobilization of permeabilized whole cell penicillin G acylase from Alcaligenes faecalis using pore matrix crosslinked with glutaraldehyde

Authors: Cheng, Shiwei; Wei, Dongzhi; Song, Qingxun; Zhao, Xiangguo

Source: Biotechnology Letters, Volume 28, Number 14, July 2006 , pp. 1129-1133(5)

Publisher: Springer

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Abstract:

The activity of penicillin G acylase from Alcaligenes faecalis increased 7.5-fold when cells were permeabilized with 0.3% (w/v) CTAB. The treated cells were entrapped by polyvinyl alcohol crosslinked with boric acid, and crosslinked with 2% (v/v) glutaraldehyde to increase the stability. The conversion yield of penicillin G to 6-aminopenicillanic acid was 75% by immobilized system in batch reaction. No activity was lost after 15 cycles and about 65% enzyme activity was retained at the end of the 31th cycle.

Keywords: Alcaligenes faecalis; Penicillin G acylase; Permeabilization; Whole cell immobilization

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10529-006-9067-x

Affiliations: 1: Email: dzhwei@ecust.edu.cn

Publication date: 2006-07-01