Influence of Water Miscible Organic Solvents on α-chymotrypsin in Solution and Immobilized on Eupergit CM

Authors: Olofsson, Linus; Söderberg, Pernilla; Nicholls, Ian

Source: Biotechnology Letters, Volume 28, Number 12, June 2006 , pp. 929-935(7)

Publisher: Springer

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Abstract:

The effects of the water-miscible organic solvents (methanol, ethanol, 1-propanol, 2-propanol, acetonitrile, N,N′-dimethylformamide and tetrahydrofuran) on the stability and catalytic activity of α-chymotrypsin (CT) immobilized on Eupergit CM were studied. Enhanced stabilities and activities were observed both as a consequence of immobilization and the presence of organic solvent, which in combination provide long term (at least 24 h) retention of activity, and up to 50-fold increase in 50% (v/v) methanol in buffer. Low quantities (20%, v/v) of acetonitrile not only prevented CT inactivation by autolysis at 20°C but also induced a significant increase in the activity of both free (six-fold) and immobilized (two-fold) CT.

Keywords: α-Chymotrypsin; Eupergit; Immobilization; Organic solvent

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10529-006-9025-7

Affiliations: 1: Email: ian.nicholls@hik.se

Publication date: 2006-06-01