Nucleotide mutations in purA gene and pur operon promoter discovered in guanosine- and inosine-producing Bacillus subtilis strains

Authors: Qian, Jiangchao; Cai, Xianpeng; Chu, Ju; Zhuang, Yingping; Zhang, Siliang

Source: Biotechnology Letters, Volume 28, Number 12, June 2006 , pp. 937-941(5)

Publisher: Springer

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Abstract:

The promoter region of the pur operon, which contains 12 genes for inosine monophosphate biosynthesis from phosphoribosylpyrophosphate, and the purA gene, encoding the adenylosuccinate synthetase, were compared among wild-type and three purine-producing Bacillus subtilis strains. A single nucleotide deletion at position 55 (relative to translation start site) in purA gene was found in a high inosine-producing strain and in a high guanosine-producing strain, which correlates with the absence of adenylosuccinate synthetase activity in these strains. Within the pur operon promoter of high guanosine-producing strain, in addition to a single nucleotide deletion in PurBox1 and a single nucleotide substitution in PurBox2, there were 4 substitutions in the flanking region of the PurBoxes and 32 nucleotide mutations in the 5′ untranslated region. These mutations may explain the purine accumulation in purine-producing strains and be helpful to the rational design of high-yield recombinant strains.

Keywords: Bacillus subtilis; Guanosine; Inosine; purA; pur operon

Document Type: Research article

DOI: http://dx.doi.org/10.1007/s10529-006-9020-z

Affiliations: 1: Email: jiangchaoqian@ecust.edu.cn

Publication date: 2006-06-01