Co-lyophilization with d-proline Greatly Enhances Peroxidase's Stereoselectivity in a Non-aqueous Medium

Authors: Yu, Ju-Hyun; Klibanov, Alexander¹

Source: Biotechnology Letters, Volume 28, Number 8, April 2006 , pp. 555-558(4)

Publisher: Springer

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Abstract:

The impact of co-lyophilizing horseradish peroxidase (HRP) with numerous amino acids and other chiral excipients on the enzyme's subsequent stereoselectivity [E(S/R)] in the sulfoxidation of thioanisole in 2-propanol was systematically investigated. While many improved the stereoselectivity of (and significantly activated) HRP, the greatest effect was observed with d-proline which enhanced the E(S/R) value by over an order of magnitude from synthetically meaningless to useful.

Keywords: asymmetric; chiral; organic solvents; sulfoxidation

Document Type: Research article

DOI: 10.1007/s10529-006-0018-3

Affiliations: 1: Email: klibanov@mit.edu