Morphological quantification of pellets in Streptomyces hygroscopicus var. geldanus fermentation broths using a flatbed scanner

Authors: O'Cleirigh C.¹; Walsh P.K.¹; O'Shea D.G.^{1, 2, 3}

Source: Biotechnology Letters, Volume 25, Number 19, October 2003 , pp. 1677-1683(7)

Publisher: Springer

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Abstract:

An image analysis technique has been developed to allow high throughput morphological characterisation of microbial fermentation broths containing spherical pellets greater than 100 mgr m in diameter. Images of stained Streptomyces hygroscopicus var. geldanus culture samples at three different inoculum levels were captured using a flatbed scanner, at a resolution of 21 mgr m per pixel (1200 dots per inch) and subsequently analysed leading to the generation of a morphological profile of each sample. The time taken for image capture and analysis of a prepared sample, containing approx. 2000 particles, was 3 min 6 s.

Keywords: flatbed scanner; image analysis; morphology; pellets; Streptomyces hygroscopicus

Language: English

Document Type: Research article

Affiliations: 1: Biochemical Engineering Research Group, National Institute for Cellular Biotechnology and School of Biotechnology, Dublin City University, Dublin 9, Ireland 2: (Fax: +353 1 7005412 3: E-mail: donal.oshea@dcu.ie)

Publication date: 2003-10-01