Expression and production of bioactive human interleukin-18 in transgenic tobacco plants
Authors: Zhang B.1; Yang Y-H.1; Lin Y-M.1; Rao Q.1; Zheng G-G.1; Wu K-F.1, 2, 3
Source: Biotechnology Letters, Volume 25, Number 19, October 2003 , pp. 1629-1635(7)
Publisher: Springer
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Abstract:
The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-
on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.
Keywords: bioactivity; human IL-18; interleukin-18; tobacco; transgenic plants
Language: English
Document Type: Research article
Affiliations: 1: National Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, P.R. China 2: (Fax: +86 22 27306542 3: E-mail: kfwu@public.tpt.tj.cn)
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