Expression and production of bioactive human interleukin-18 in transgenic tobacco plants

Authors: Zhang B.¹; Yang Y-H.¹; Lin Y-M.¹; Rao Q.¹; Zheng G-G.¹; Wu K-F.^{1, 2, 3}

Source: Biotechnology Letters, Volume 25, Number 19, October 2003 , pp. 1629-1635(7)

Publisher: Springer

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Abstract:

The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN- ggr on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g^-1 tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.

Keywords: bioactivity; human IL-18; interleukin-18; tobacco; transgenic plants

Language: English

Document Type: Research article

Affiliations: 1: National Laboratory of Experimental Hematology, Institute of Hematology & Blood Disease Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, P.R. China 2: (Fax: +86 22 27306542 3: E-mail: kfwu@public.tpt.tj.cn)

Publication date: 2003-10-01