Rapid 2,2-bicinchoninic-based xylanase assay compatible with high throughput screening^**

Authors: Kenealy W.R.^{1, 2, 3}; Jeffries T.W.¹

Source: Biotechnology Letters, Volume 25, Number 19, October 2003 , pp. 1619-1623(5)

Publisher: Springer

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Abstract:

High-throughput screening requires simple assays that give reliable quantitative results. A microplate assay was developed for reducing sugar analysis that uses a 2,2 prime -bicinchoninic-based protein reagent. Endo-1,4- bgr -d-xylanase activity against oat spelt xylan was detected at activities of 0.002 to 0.011 IU ml^-1. The assay is linear for sugar concentrations from 0 to 86 mgr g ml^-1 and can also be used to assay protein concentrations (0 to 143 mgr g ml^-1) on the same plate. A variety of temperatures and pH conditions can be used and, after incubation, the assay requires only one detection reagent and one heating step.

Keywords: bicinchoninic acid; high throughput; xylanase

Language: English

Document Type: Research article

Affiliations: 1: Institute for Microbial and Biochemical Technology, USDA Forest Service, Forest Products Laboratory, One Gifford Pinchot Drive, Madison, WI 53726-2398, USA 2: (Fax: +608 231-9262 3: E-mail: bkenealy@fs.fed.us)

Publication date: 2003-10-01