Bioreactor cultivation of Escherichia coli for production of recombinant penicillin G amidase from Alcaligenes faecalis

Authors: Deak P.M.1; Lutz-Wahl S.1; Bothe H.1; Fischer L.1, 2, 3

Source: Biotechnology Letters, Volume 25, Number 5, March 2003 , pp. 397-400(4)

Publisher: Springer

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Abstract:

The penicillin G amidase (PGA) from Alcaligenes faecalis, which has interesting properties for use in combinatorial biochemistry, was produced by recombinant expression in Escherichia coli. The corresponding gene was cloned into a multicopy vector under the strict regulatory control of the rhamnose inducible promoter. Cells were grown in a synthetic minimal medium in a bioreactor (5 l working vol.), and production of PGA was induced by repeated addition of the inducer rhamnose, that served also as a carbon source. The fermentation yield was about 4500 units PGA activity per liter of culture medium.

Keywords: Alcaligenes faecalis; fermentation; penicillin G amidase; recombinant protein production; rhamnose inducible promoter

Language: English

Document Type: Research article

Affiliations: 1: Lehrstuhl für Biotechnologie, Institut für Lebensmitteltechnologie, Universität Hohenheim, Emil-Wolff-Straße 14, 70599 Stuttgart, Germany 2: (Fax: +49-711-459-4267 3: E-mail: lfischer@uni-hohenheim.de)

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