Methanol determination in Pichia pastoris cultures by flow injection analysis

Authors: Almuzara C.¹; Cos O.²; Baeza M.¹; Gabriel D.²; Valero F.³

Source: Biotechnology Letters, Volume 24, Number 5, March 2002 , pp. 413-417(5)

Publisher: Springer

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Abstract:

An automated reverse flow injection analysis (r-FIA) system using stop-flow technique for quantifying methanol based on the enzymatic reactions of alcohol oxidase and peroxidase was developed. The system permitted methanol analysis in a linear range of 0.006-0.1 g methanol l^−1 without external dilution, and with a sampling frequency of 12 analyses per hour, with a relative standard deviation of 1.16%. The analyser was validated analysing samples from a Pichia pastoris fermentation producing a heterologous protein.

Keywords: alcohol oxidase; flow injection analysis; methanol; peroxidase; Pichia pastoris

Language: English

Document Type: Regular paper

Affiliations: 1: Escola Universitària Politècnica del Medi Ambient, 08100 Mollet del Vallés, Spain 2: Departament d'Enginyeria Química, ETSE, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain 3: E-mail: francisco.valero@uab.es)

Publication date: 2002-03-01